![]() It is possible to simultaneously visualise both small and large cells on the FSC axis if you display the data in log form. If you’re only interested in the leukocytes then this isn’t really a problem per se, and simply reflects the limitation of the machine to display such a large range of cell sizes on a linear scale. Without knowing these factors I would predict that your FSC is slightly too big and the stomal cells are causing the “problem”. What was the ratio of leukocytes to stromal cells? (if 1:100 then this is obviously predictable/acceptable, but if it is, say, 1:10 then it’s possible that you could be losing blasting leukocytes off the axis).Īlso, are you looking at the data on a log or linear scale? ![]() This is the FSC vs SSC plot, correct? Not FSC vs a fluorescence parameter? The cells can only be “too big” on FSC and SSC, but they can be too small on fluorescence channels due to compensation or baseline restore.ĭid you use a BD machine when running the samples? (my experience and comments are applicable to BD/DiVA, not necessarily other machines). ![]()
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